Year 3

This is a new cell lines award which involves the derivation of new human embryonic stem cell lines from human blastocysts. In this reporting period we have generated fifteen new hESC lines. In particular, we have generated two new disease hESC lines for Patau syndrome (Trisomy 31) and an additional disease hESC line for Down Syndrome (Trisomy 21). We have also generated a comprehensive collection of standard operating procedures (SOPs) for the current good manufacturing of new hESC lines at our institution.