Microenvironment for hiPSC-derived pacemaking cardiomyocytes

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Grant Application Details

Application Title:

Microenvironment for hiPSC-derived pacemaking cardiomyocytes

Public Abstract:
Research Objective

This proposal investigates the effects of the microenvironment on the development and maintenance of pacemaking function in human induced pluripotent stem cell (hiPSC)-derived cardiomyocytes.


Pacemaking function of hiPSC-derived cardiomyocytes is lost over time. Sustainability of pacemaking function of these cells is critical for engineering an biopacemaker from the patient's own cells.

Major Proposed Activities

  • Determine the effects of matrix scaffolds on the differentiation and maintenance of pacemaking function in hiPSC-derived cardiomyocytes.
  • Determine the appropriate hiPSC-derived cardiac cells to be subjected to the microenvironment for efficient yield of pacemaking hiPSC-derived cardiomyocytes.
  • Induce vascularization in tissue constructs in small animals to sustain pacemaking tissue construct.
  • Test sustainability of a functional pacemaking tissue construct in a small animal model.
Statement of Benefit to California:
Over 350,000 patients a year in the U.S. require an electronic pacemaker to restore their heart rhythm. The annual healthcare burden amounts to $20 billion. Repeated surgeries to replace battery and electrical parts generate additional costs and suffering for the patients. A biopacemaker engineered from human stem cell-derived pacemaking cells can overcome problems associated with electronics and improve the quality of life for the pacemaker recipient while reducing cumulative health care costs.