Name:
Institution:
Type:
PI
Grant Award Details
Establishment of a novel approach to systematically study the dynamic organization of protein complexes in stem cells
Grant Type:
Grant Number:
DISC0-14405
Project Objective:
- To develop a method for simultaneous identification and characterization of multiple interactions between proteins within functional complexes (Prod-seq), and between DNA associated-proteins and the genome (WhIP-seq). The method will be applied to investigate poly comb group interactions in iPSC-derived neural progenitors and neurons.
Investigator:
Human Stem Cell Use:
iPS Cell
Award Value:
$1,515,601
Status:
Pre-Active
Grant Application Details
Application Title:
- Establishment of a novel approach to systematically study the dynamic organization of protein complexes in stem cells
Public Abstract:
Research Objective
We focus on hiPSCs pluripotency and neurodifferentiation to develop a novel framework to allow simultaneous identification of multiple interactions between proteins and between proteins and the genome
Impact
Our framework will allow high-throughput queries of the organization and functionality of proteins and shift the focus towards unprecedented, multi-dimensional studies of the cellular complexity.
Major Proposed Activities
- Establish two new complementary tools (Prod-seq and WhIp-seq) to catalog protein interactions, genomic binding and abundance.
- Employ the new tools (Prod-seq and WhIP-seq) to study the polycomb group complex (PcG) members and their molecular interactions in hiPSCs.
- Perform Prod-seq and WhIP-seq on hiPSCs-derived cortical NPCs and neurons.
- Identify PcG configuration and interaction patterns during hiPSCs neurodifferentiation and validate the results by biochemical experiments and by comparing our findings to previously published data.
Statement of Benefit to California:
The successful completion of our project will pave the road towards new preventative strategies, treatments, and cures for diseases applicable to a variety of ethnic groups, and will therefore benefit the State of California and its highly ethnically diverse citizens. As we focus our efforts on induced pluripotent stem cells (iPSCs), our approach has the potential to advance the understanding of the physiology and disease using samples obtained from individuals from various genetic backgrounds.
