Transcription factor (TF), protein that controls the rate of transcription of genetic information from DNA to messenger RNA, networks are important in cell fate decision in mammalian development and adult tissue homeostasis but are riddled with mutations in diseases. Recent technological advances, in particular large-scale genome-wide approaches, single-cell methodologies, live-cell imaging, and genome editing, are emerging as important technologies in unraveling TF network biology. We examine how these emerging technologies facilitate new ways to understand the complex nature of the TF network.