Project Objective: Research Insights


Human Immune System Mouse models as preclinical platforms for stem cell derived grafts

A major obstacle to stem cell based therapies is the immune response of the patient to stem cell derived tissue, which can be recognized as foreign and attacked by the patient’s immune system. T cells orchestrate immune responses and are “educated” about self versus foreign in an organ called the thymus. It may be possible […]

Engineered immune tolerance by Stem Cell-derived thymic regeneration

Stem cell therapies have the potential to transform medicine by allowing the regeneration of tissues or organs damaged by disease or trauma. In order for stem cell therapies to proceed, it will be essential that the regulation of immune responses to the stem cell derived tissues be achieved. While the function of the immune system […]

Inactivating NK cell reactivity to facilitate transplantation of stem cell derived tissue

One of the great promises of stem cell research is that it will one day be possible to prepare replacement cells or organs from stem cells such as embryonic stem cells, which can be transplanted to patients to substitute for diseased or defective patient tissues or organs. Unfortunately, the immune system reacts against, and rejects, […]

Regulatory T cell induced tolerance to ESC transplantation

The goals of this proposal are to utilize cell populations known to control immune reactions termed regulatory T cells and study their ability to protect embryonic stem cells (ESC) from immune rejection. Much has been learned about the control of immune reactions where it has been found that a variety of different factors control excessive […]

Role of HLA in neural stem cell rejection using humanized mice

One of the key issues in stem cell transplant biology is solving the problem of transplant rejection. Despite over three decades of research in human embryonic stem cells, little is known about the factors governing immune system tolerance to grafts derived from these cells. In order for the promise of embryonic stem cell transplantation for […]

Role of HLA in neural stem cell rejection using humanized mice

One of the key issues in stem cell transplant biology is solving the problem of transplant rejection. Despite over three decades of research in human embryonic stem cells, little is known about the factors governing immune system tolerance to grafts derived from these cells. In order for the promise of embryonic stem cell transplantation for […]

Engineering Embryonic Stem Cell Allografts for Operational Tolerance

Stem cells, like all transplants not derived from an identical twin, are subject to scrutiny by the immune system and, without medical interventions that suppress the immune system, are usually killed after transplantation. However, rare exceptions to this rule exist because a small fraction of transplant patients has been able to maintain their transplant in […]

Engineering Thymic Regeneration to Induce Tolerance

A healthy immune system produces T cells that can recognize and react against foreign molecules (antigens) to protect against infection, while leaving normal host cells with “self antigens” undamaged. All T cells are produced in the thymus from blood stem cells that migrate from the bone marrow. “Tolerant” T cells are those that have been […]

Donor natural killer (NK) cells as “veto” cells to promote donor-specific tolerance

A major issue in the use of stem cells or in organ transplantation in general is the need to overcome graft rejection. Unfortunately, the only means currently available involves the use of systemic immunosuppression which leaves the recipient at risk for opportunistic infections. This proposal will seek to use the donor’s immune cells to prevent […]

Application of Tolerogenic Dendritic Cells for Hematopoietic Stem Cell Transplantation

The immune system protects us from invading pathogens, but has to be kept in check to prevent harmful responses to our own tissues. Unique immune “suppressor” cells have been recently characterized that prevent harmful responses to our own cells and proteins. We have recently identified unique populations of white blood cells, called dendritic cells that […]