Grant Award Details
Determine if human hepatocyte progenitor cells, which exist in the normal adult liver, can be maintained and expanded in vitro while maintaining in vivo regenerative capacity.; expected outcome is a liver-derived, ex vivo expandable human hepatocyte progenitor cell population (HPCs) for treating liver damage/disease
Grant Application Details
- Preclinical development of human hepatocyte progenitor cells for cell therapy
Determine if human hepatocyte progenitor cells, which exist in the normal adult liver, can be maintained and expanded in vitro while maintaining in vivo regenerative capacity.
Cell transplantation therapy can be an effective alternative treatment for severe liver diseases to liver transplantation, which is severely limited by the lack of available donor organs.
Major Proposed Activities
- Characterize human pericentral hepatocytes and their niche in normal adult human liver
- Determine if human pericentral hepatocytes function as progenitor cells in a humanized mouse liver model
- Compare the regenerative capacity of human HPCs with mature hepatocytes
- Determine the optimum in vitro conditions for maintaining and expanding human HPCs
- Examine whether endothelial cells promote in vitro expansion of human HPCs
- Assess the liver repopulating capability of long-term culture expanded HPCs
Cellular therapy for severe liver disease in the form of hepatocyte transplantation is effective alternative to whole organ transplantation. However, its usage is limited by the severe shortage of healthy primary human hepatocytes. The potential to generate patient-specific sources of hepatocytes from HPCs for cellular therapy would address an immense unmet clinical need.