Non-invasive live imaging of stem cell signature metabolic states

Funding Type: 
Basic Biology V
Grant Number: 
RB5-07458
Investigator: 
Type: 
PI
Award Value: 
$540,480
Stem Cell Use: 
Embryonic Stem Cell
Adult Stem Cell
Status: 
Active
Public Abstract: 

Stem cells in tissues exhibit unique metabolic states that could distinguish them from other more specialized cells. Using a new type of microscopy we can image the metabolic states of cells in living tissues and, therefore, attempt to identify stem cell populations. Because stem cells can give rise to tumors that are metabolically very different from normal tissues, being able to identify cells that are about to form tumors could be immensely useful for early cancer diagnosis. Such methods can also be used to study embryonic stem cells as a model of early development, thus providing new information about early development and the risks to the embryo from the environment. along a continuum of oxidative phosphorylation and glycolysis that can be used to distinguish them from differentiated daughters and from surrounding niche cells. If successful these methods could be used in clinical settings for better diagnoses of human disease.

Statement of Benefit to California: 

A goal of Prop 71 is to take basic stem cell research to clinical application. Disability and loss of earning power and personal freedom resulting from a disease are devastating, creating a financial burden for the State in addition to suffering caused to patients and families. Therapies using human stem cells have the potential to change millions of lives. Using hES cells as disease models will help us understand the underlying causes of disease and aid in the development of drugs to treat them. Our research is aimed at identifying key changes in stem cells in normal development and disease. These studies could aid in understanding normal developemnt, risks to the embryo, as well as early cancer detection. Anticipated benefits of our research include:
1.Development of new cell-based treatments for diseases
2.Improved methods for understanding development and risks to the embryo
3.New methods for detecting effects of toxicants in the environment/workplace
4.Development of new methods for developing drugs for treating disease
5.Transfer of new technologies and IP to the public realm with resulting revenues coming to the state
6.Creation of new biotechnology companies based on new IP
7.Creating research teams with a competitive edge for obtaining out of state funding
8.Creation of new jobs in the biotechnology sector.

It is anticipated that, in the long term, the return to the State in terms of revenue, health benefits for its Citizens and job creation will be significant.

Progress Report: 

During the last reporting period we have continued to develop technology that allows non-invasive real time imaging of living stem cells in their natural state and can allow stem cells to be distinguished from their most closely-related differentiated derivatives. The methodology has been developed such that we will soon be able to measure the absolute concentration of specific metabolites within living cells. This in turn will allow the concentrations of such metabolites to be compared between cell types, thus enabling comparative studies of stem cells and closely related daughter cells and stem cells and their in vitro counterparts. In the long run these studies should provide new information about how pluripotent stem cells normally develop and how their development may be impaired by environmental factors. This information in turn could help us understand more about normal human development and how it can all too often go wrong. Studies of adult stem cells in tissues could also allow us to understand what factors affect normal growth of stem cells in tissues and how that process can go awry in the development of cancer.