Name:
Institution:
Type:
PI
Grant Award Details
Identifying roadblocks to neural stem cell transplantation into human tissues.
Grant Type:
Grant Number:
DISC0-14429
Project Objective:
- Developing novel tools to understand the trajectory and regulation of neural stem cell differentiation in both primary human tissues and in iPS-derived organoids - with particular focus on the 2 NSC types in the human neocortex, truncated and outer radial glia, and also identify roadblocks to the successful transplantation of iPS derived neurons into the adult brain.
Investigator:
Disease Focus:
Neurological Disorders
Human Stem Cell Use:
Adult Stem Cell
iPS Cell
Award Value:
$1,551,394
Status:
Pre-Active
Grant Application Details
Application Title:
- Identifying roadblocks to neural stem cell transplantation into human tissues.
Public Abstract:
Research Objective
We will generate a comprehensive map of human neural stem cell differentiation profiles that will serve as a reference for enhancing neural stem cell-based therapies.
Impact
Our project will develop improved protocols for human neural stem cells differentiation, enhancing the fidelity, safety and robustness of future cell therapies.
Major Proposed Activities
- Establish quantitative map of neural stem cell differentiations to serve as a reference.
- Determine how radial glia neural stem cell differentiation is impacted by genetic or environmental perturbation.
- Quantitatively compare neural stem cell differentiation in human and cerebral organoid model.
- Determine mechanisms underlying neural stem cell differentiation in human developing brain tissue.
- Compare neural stem cell survival in mouse brain tissue.
Statement of Benefit to California:
Californians asked CIRM to support stem cell research towards treatments for brain disorders by earmarking 25% funds for CNS projects. Cell therapy applications in the nervous system have the potential to address CNS disorders including Epilepsy, Stroke, and Neurodegenerative Disorders. However, neural stem cell transplantation is inefficient and poorly understood in human brain tissue. Our project directly addresses these limitations using a combination of genetic and cellular approaches.
