Generation of human airway stem cells by direct transcriptional reprogramming for disease modeling and regeneration
Grant Award Details
Grant Type:
Grant Number:
DISC1-10475
Investigator(s):
Disease Focus:
Human Stem Cell Use:
Cell Line Generation:
Award Value:
$238,408
Status:
Closed
Progress Reports
Reporting Period:
Year 2
Grant Application Details
Application Title:
Generation of human airway stem cells by direct transcriptional reprogramming for disease modeling and regeneration
Public Abstract:
Research Objective
We will generate human airway stem cells by direct transcriptional reprogramming of fibroblasts. We will use these induced airway stem cells to model motile cilia disease in a dish.
Impact
Generating airway stem cells through reprogramming will create a scalable and editable cell line from which we can derive airway epithelium, thus enabling airway disease modeling and drug screening.
Major Proposed Activities
We will generate human airway stem cells by direct transcriptional reprogramming of fibroblasts. We will use these induced airway stem cells to model motile cilia disease in a dish.
Impact
Generating airway stem cells through reprogramming will create a scalable and editable cell line from which we can derive airway epithelium, thus enabling airway disease modeling and drug screening.
Major Proposed Activities
- Aim 1: Direct reprogramming of fibroblasts into human airway stem cells
- Aim 2: Modeling motile cilia disease in reprogrammed human airway stem cells
Statement of Benefit to California:
More than 10% of Californians suffer from lung diseases such as COPD or asthma. Our proposed studies will attempt to directly convert skin cells into lung stem cells, facilitating the modeling of airway diseases in a dish. Disease modeling will lead to a deeper understanding of the cellular basis of airway diseases which could lead to novel drugs and regenerative therapies in the future, benefiting the people of California and beyond.
