Ex vivo fate mapping of human lung stem cell plasticity in fibrotic disease

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Grant Award Details

Grant Number:
DISC0-14460
Investigator(s):
Human Stem Cell Use:
Award Value:
$1,625,998
Status:
Active

Grant Application Details

Application Title:

Ex vivo fate mapping of human lung stem cell plasticity in fibrotic disease

Public Abstract:
Research Objective

This proposal will design new models to study behavior that is specific to human lung stem cells, and screen for drugs that can target abnormal stem cells in fibrotic disease.

Impact

This study will generate potential new therapeutic approaches to fibrotic lung diseases such as idiopathic pulmonary fibrosis.

Major Proposed Activities

  • Development of a combinatorial viral lineage tracing tool kit in mouse lung slices
  • Utilizing combinatorial viral lineage tracing in human lung slices to define human lung stem cell lineage trajectory after fibrotic injury
  • Identification of candidate compounds that can block abnormal lung stem cell differentiation
  • Development of fate mapping tools to study lung stem cells in diseased human tissue
  • Identification of candidate compounds that can reverse abnormal stem cell differentiation in the fibrotic lung
Statement of Benefit to California:
Our proposal will seek new ways to target stem cells in fibrotic lung diseases that affect Californians. We will generate new approaches to study lung stem cells that could serve as a platform to discover drugs that will improve stem cell health in lung disease.