Name:
Institution:
Type:
PI
Grant Award Details
Ex vivo fate mapping of human lung stem cell plasticity in fibrotic disease
Grant Type:
Grant Number:
DISC0-14460
Project Objective:
- Develop a novel fate mapping platform to study the lineage trajectory of hAT2s, a major human lung stem cell population that is lost during fibrosis, ex vivo in precision cut lung slices (PCLS) derived from diseased human lungs.
Investigator:
Disease Focus:
Lung Disease, Fibrosis
Respiratory Disorders
Human Stem Cell Use:
Adult Stem Cell
Award Value:
$1,625,998
Status:
Pre-Active
Grant Application Details
Application Title:
- Ex vivo fate mapping of human lung stem cell plasticity in fibrotic disease
Public Abstract:
Research Objective
This proposal will design new models to study behavior that is specific to human lung stem cells, and screen for drugs that can target abnormal stem cells in fibrotic disease.
Impact
This study will generate potential new therapeutic approaches to fibrotic lung diseases such as idiopathic pulmonary fibrosis.
Major Proposed Activities
- Development of a combinatorial viral lineage tracing tool kit in mouse lung slices
- Utilizing combinatorial viral lineage tracing in human lung slices to define human lung stem cell lineage trajectory after fibrotic injury
- Identification of candidate compounds that can block abnormal lung stem cell differentiation
- Development of fate mapping tools to study lung stem cells in diseased human tissue
- Identification of candidate compounds that can reverse abnormal stem cell differentiation in the fibrotic lung
Statement of Benefit to California:
Our proposal will seek new ways to target stem cells in fibrotic lung diseases that affect Californians. We will generate new approaches to study lung stem cells that could serve as a platform to discover drugs that will improve stem cell health in lung disease.
