CSUN-UCLA Bridges to Stem Cell Research
Grant Award Details
Grant Type:
Grant Number:
TB1-01183
Investigator(s):
Name:
Type:
PI
Award Value:
$3,814,078
Status:
Closed
Progress Reports
Reporting Period:
Year 3
Reporting Period:
Year 4
Reporting Period:
Year 5
Reporting Period:
Year 6
Reporting Period:
Year 7
Grant Application Details
Application Title:
Bridges to Stem Cell Research
Public Abstract:
The Bridges to Stem Cell Research Training Program will provide a practical laboratory training experience in stem cell biology with integrated educational seminars and mentored guidance for highly qualified and culturally diverse senior undergraduate and Master’s level students. Our internship-host institution provides mentors who are world-leaders in stem cell research. There is a great scientific variety of available hands-on training environments in embryonic, induced pluripotent, and adult stem cell biology, spanning the basic to translational investigative spectrum. Our partnership achieves the major Bridges Program objectives including: 1) training laboratory personnel in current stem cell research techniques, policies, and ethics, and 2) facilitating the entry of an ethnically and culturally diverse student population into the emerging world of stem cell biology and regenerative medicine. Ten Bridges trainees will study the latest advances in stem cell biology and will present their own work in a setting in which they can obtain constructive feedback. They will interact with their peers in formal and informal forums and will meet leaders in the field. Bridges internships will be 1 year in duration for graduate students and 9 months for undergraduate students and will take place in screened and selected internship-host labs. The vast majority of intern time will be spent on laboratory research. Working with faculty, researchers, and staff technicians, Bridges interns will be taught stem cell and essential analysis techniques such as microscopy, cell sorting, and good laboratory practices (GLP) in the internship-host lab and affiliated cores. This hands-on experience will be supplemented by participation in a biweekly Stem Cell Journal Club, a weekly stem cell seminar series, a yearly International Stem Cell Symposium, and by informal mentoring by host-institution faculty who have an active role in the student's education by functioning as advisor, teacher, and collaborator during the internship program. A key component is trainee mentoring that is designed to not only provide a supportive environment for learning and discovery, but to monitor and evaluate a trainee's progress through the program at both the home and internship-host institution. Our inter-institutional training program will provide an opportunity for engaged, interested, and successful trainees to gain the necessary skills and qualifications to springboard into careers in stem cell research that span the spectrum, from basic studies to translational approaches, in academia and industry. The home institution has a long history of successfully preparing underrepresented minority students for doctoral study in science and technology. This program is expected to build on this track record by contributing significantly to the number of minority students preparing for careers in stem cell biology and regenerative medicine.
Statement of Benefit to California:
The Bridges to Stem Cell Research Program will train students in world-leading host institution labs for technical positions in stem cell research in academia and industry. Interns will come from a culturally and ethnically diverse applicant pool, increasing underrepresented groups engaged in stem cell research. The grantee institution was recently ranked among the top five by the NSF for MS degree holders to earn a subsequent PhD degree, which likely will add to the diversity of stem cell teachers and researchers at California institutions. Our training program will also provide scientists and technical staff for California's biotechnology, pharmaceutical, and stem cell companies, whose success will propel hiring and increased economic prosperity for the state. The host institution supports a scientific enterprise of $900M+ in extramural funding (2007) with ~$46M in CIRM grants and is one of the largest State employers with economic activity generating $1.2B annually in taxes. Each dollar of taxpayer investment generates almost $15 in economic activity. The grantee institution also generates $900M+ million for the regional economy. This impact sustains more than 13,500 jobs and generates $57M per year in tax revenue. CIRM trainees will have a tangible health and economic impact for California, its academic institutions and biotechnology, pharmaceutical, and stem cell companies, and the rest of the nation as California and its citizens move forward with personalized medicine during the 21st century.
Publications
- Immunity (2017): 25-Hydroxycholesterol Protects Host against Zika Virus Infection and Its Associated Microcephaly in a Mouse Model. (PubMed: 28314593)
- Stem Cell Res (2016): Alcohol-induced suppression of KDM6B dysregulates the mineralization potential in dental pulp stem cells. (PubMed: 27286573)
- Cell Metab (2016): alpha-Ketoglutarate Accelerates the Initial Differentiation of Primed Human Pluripotent Stem Cells. (PubMed: 27476976)
- Proc Natl Acad Sci U S A (2017): Atomic structure of a toxic, oligomeric segment of SOD1 linked to amyotrophic lateral sclerosis (ALS). (PubMed: 28760994)
- Elife (2017): BMPs direct sensory interneuron identity in the developing spinal cord using signal-specific not morphogenic activities. (PubMed: 28925352)
- PLoS One (2012): Characterization and therapeutic potential of induced pluripotent stem cell-derived cardiovascular progenitor cells. (PubMed: 23056209)
- Nature (2016): Chromosome conformation elucidates regulatory relationships in developing human brain. (PubMed: 27760116)
- Nat Cell Biol (2017): Control of intestinal stem cell function and proliferation by mitochondrial pyruvate metabolism. (PubMed: 28812582)
- Stem Cell Reports (2014): Defining the role of oxygen tension in human neural progenitor fate. (PubMed: 25418722)
- Stem Cell Reports (2018): Deriving Dorsal Spinal Sensory Interneurons from Human Pluripotent Stem Cells. (PubMed: 29337120)
- Circ Res (2014): Developmental heterogeneity of cardiac fibroblasts does not predict pathological proliferation and activation. (PubMed: 25037571)
- Stem Cell Res Ther (2017): Differentiation of RPE cells from integration-free iPS cells and their cell biological characterization. (PubMed: 28969679)
- Cell (2018): The Dynamic Landscape of Open Chromatin during Human Cortical Neurogenesis. (PubMed: 29307494)
- Data Brief (2017): Engineered HA hydrogel for stem cell transplantation in the brain: Biocompatibility data using a design of experiment approach. (PubMed: 27995155)
- PLoS One (2012): Engineering HIV-1-resistant T-cells from short-hairpin RNA-expressing hematopoietic stem/progenitor cells in humanized BLT mice. (PubMed: 23300932)
- Cell Metab (2015): Epigenome-wide association of liver methylation patterns and complex metabolic traits in mice. (PubMed: 26039453)
- Nat Cell Biol (2018): ERBB3 and NGFR mark a distinct skeletal muscle progenitor cell in human development and hPSCs. (PubMed: 29255171)
- Cell Rep (2020): Foxp1 Regulates Neural Stem Cell Self-Renewal and Bias Toward Deep Layer Cortical Fates. (PubMed: 32049024)
- Elife (2017): Glucose inhibits cardiac muscle maturation through nucleotide biosynthesis. (PubMed: 29231167)
- Cell Stem Cell (2015): GPI-80 defines self-renewal ability in hematopoietic stem cells during human development. (PubMed: 25465114)
- Blood (2013): HIV-1 Infection of hematopoietic progenitor cells in vivo in humanized mice. (PubMed: 23886835)
- Nat Commun (2019): Human evolved regulatory elements modulate genes involved in cortical expansion and neurodevelopmental disease susceptibility. (PubMed: 31160561)
- J Immunol (2014): Human Lymphoid Development in the Absence of Common gamma-Chain Receptor Signaling. (PubMed: 24771849)
- Cell Stem Cell (2020): A Human Skeletal Muscle Atlas Identifies the Trajectories of Stem and Progenitor Cells across Development and from Human Pluripotent Stem Cells. (PubMed: 32396864)
- Nat Neurosci (2021): Identification of neural oscillations and epileptiform changes in human brain organoids. (PubMed: 34426698)
- Stem Cell Res (2016): Identifying gene expression modules that define human cell fates. (PubMed: 27108395)
- Proc Natl Acad Sci U S A (2013): Long-term in vivo monitoring of mouse and human hematopoietic stem cell engraftment with a human positron emission tomography reporter gene. (PubMed: 23319634)
- Stem Cell Reports (2018): Loss of MECP2 Leads to Activation of P53 and Neuronal Senescence. (PubMed: 29742391)
- Nat Protoc (2012): Measuring energy metabolism in cultured cells, including human pluripotent stem cells and differentiated cells. (PubMed: 22576106)
- Hum Gene Ther (2013): Modification of hematopoietic stem/progenitor cells with CD19-specific chimeric antigen receptors as a novel approach for cancer immunotherapy. (PubMed: 23978226)
- Neuro Oncol (2018): A molecular cascade modulates MAP1B and confers resistance to mTOR inhibition in human glioblastoma. (PubMed: 29136244)
- Dev Cell (2015): Notch activity modulates the responsiveness of neural progenitors to sonic hedgehog signaling. (PubMed: 25936505)
- CRISPR J (2021): Optimizing Integration and Expression of Transgenic Bruton’s Tyrosine Kinase for CRISPR-Cas9-Mediated Gene Editing of X-Linked Agammaglobulinemia. (PubMed: 33876953)
- Sci Transl Med (2021): Patient-derived glial enriched progenitors repair functional deficits due to white matter stroke and vascular dementia in rodents. (PubMed: 33883275)
- Mol Ther Nucleic Acids (2016): Reactivating Fetal Hemoglobin Expression in Human Adult Erythroblasts Through BCL11A Knockdown Using Targeted Endonucleases. (PubMed: 28131278)
- Nat Cell Biol (2023): Regenerating human skeletal muscle forms an emerging niche in vivo to support PAX7 cells. (PubMed: 37919520)
- PLoS One (2015): REST/NRSF Knockdown Alters Survival, Lineage Differentiation and Signaling in Human Embryonic Stem Cells. (PubMed: 26690059)
- Cell Rep (2017): Self-Organized Cerebral Organoids with Human-Specific Features Predict Effective Drugs to Combat Zika Virus Infection. (PubMed: 29020636)
- Neuron (2019): A Single-Cell Transcriptomic Atlas of Human Neocortical Development during Mid-gestation. (PubMed: 31303374)
- Cell Stem Cell (2016): A Single CRISPR-Cas9 Deletion Strategy that Targets the Majority of DMD Patients Restores Dystrophin Function in hiPSC-Derived Muscle Cells. (PubMed: 26877224)
- Sci Rep (2017): A small molecule screen to identify regulators of let-7 targets. (PubMed: 29162914)
- Biomaterials (2016): Systematic optimization of an engineered hydrogel allows for selective control of human neural stem cell survival and differentiation after transplantation in the stroke brain. (PubMed: 27521617)
- Cell Rep (2017): In Vivo Human Somitogenesis Guides Somite Development from hPSCs. (PubMed: 28178531)
- Stem Cell Reports (2018): X Chromosome Dosage Influences DNA Methylation Dynamics during Reprogramming to Mouse iPSCs. (PubMed: 29681539)
- Cell (2014): X chromosome reactivation dynamics reveal stages of reprogramming to pluripotency. (PubMed: 25525883)
