This article presents data related to the research article “Systematic optimization of an engineered hydrogel allows for selective control of human neural stem cell survival and differentiation after transplantation in the stroke brain” (P. Moshayedi, L.R. Nih, I.L. Llorente, A.R. Berg, J. Cinkornpumin, W.E. Lowry et al., 2016) and focuses on how well the hydrogel works in a mouse model. the previous paper showed its usefulness for growing stem cells in culture, and here we show it can be injected into stroke damaged areas of mouse brains without causing further damage. Additionally, the hydrogel continues to support and release the growth factors the stem cells need to grow and repair the brain damage after stroke and transplantation. Injections containing hydrogel and stem cells versus stem cells alone showed better growth and transplantation of stem cells in the stroke damaged area of the mouse brains.