Grant Award Details
- The objective of the CIRM Shared Lb is to maintain a resource to the scientific community for conducting pluripotent stem cell research.
Grant Application Details
- Collaborative Laboratory for Human Embryonic Stem Cell Research
We are proposing to expand our “safe haven” human embryonic stem cell laboratory to accommodate the enormous interest in scientific research in this field, and to provide an environment that is conducive to the goals of the CIRM’s Strategic Plan. Our collaborative Shared Laboratory will support the research of all of our institution’s many stem cell researchers, including the new investigators who have been recently approved for funding under the CIRM’s SEED grant program. In addition, we will cooperate will neighboring institutions to minimize overlap in strategic technological areas and maximize the value of CIRM’s investment in our scientific community. The scientists in our program will share their special expertise in the areas of human ES cell derivation and molecular analysis.
All aspects of the Shared Laboratory will be directed by the Program Director, a well-established senior stem cell scientist who has experience in laboratory design and management of large groups of researchers. An Oversight Committee, composed of leading scientists, ethicists, and institution management will meet regularly to monitor and oversee the activities of the Laboratory.
We will also offer a series of Basic and Advanced Stem Cell Techniques Courses on behalf of our local scientific community. A Public Education Program will provide non-scientists with the opportunity to have hands-on experience with hESC research. Alumni from the courses will have access to an interactive web-based discussion group, and will meet once a year to share their scientific discoveries and insights. By closely collaborating with other California institutions, we plan to take full advantage of CIRM’s investment in stem cell research and speed the translation of stem cell-based therapies to the clinic
Californians are a large and diverse population that poses unique challenges for the future of medical care. Fortunately, California has a tradition of taking the lead in technology and medical breakthroughs and following through from the first idea to the final product. A major goal for California’s supporters of stem cell research is development of stem cell-based products that have medical use, and the mandate for the research community is to provide the best possible fundamental information to help guide clinical applications. We have already laid the groundwork for research that encompasses both federally approved and non-approved human embryonic stem cells (hESC) by establishing a privately funded safe haven stem cell laboratory and founding a non-profit IRB-approved storage facility for excess embryos that have been donated for research. We have created an informational website and generated the largest worldwide public database of molecular information from our analyses of approved and non-approved hESC. We have been offering hands-on comprehensive courses in hESC technologies for three years, and have launched popular programs for scientific and ethical discussions that are regularly attended by hundreds of Californians. We propose to build on this foundation and expand our breadth and depth in stem cell biology through creation of a CIRM-supported collaborative Shared Laboratory and Stem Cell Techniques Course. We have designed this program to maximize benefit to both our own and neighboring institutions, to enhance collaborative interaction and open doors for the next generation of stem cell scientists. The Laboratory and Course will be a magnet for other researchers to contribute their own expertise, which will leverage the power of the California stem cell community. The program will be a springboard to new commercial ventures and will speed the development of clinical applications for stem cells that will benefit all Californians.
- Proc Natl Acad Sci U S A (2017) Probing the lithium-response pathway in hiPSCs implicates the phosphoregulatory set-point for a cytoskeletal modulator in bipolar pathogenesis. (PubMed: 28500272)
- Stem Cell Reports (2017) iPSCORE: A Resource of 222 iPSC Lines Enabling Functional Characterization of Genetic Variation across a Variety of Cell Types. (PubMed: 28392216)
- Stem Cells (2017) Spontaneous Single-Copy Loss of TP53 in Human Embryonic Stem Cells Markedly Increases Cell Proliferation and Survival. (PubMed: 27888558)
- Nat Commun (2016) Whole-genome mutational burden analysis of three pluripotency induction methods. (PubMed: 26892726)
- Zoo Biol (2016) Rewinding the process of mammalian extinction. (PubMed: 27142508)
- Genome Res (2015) Dynamic changes in replication timing and gene expression during lineage specification of human pluripotent stem cells. (PubMed: 26055160)
- Expert Opin Biol Ther (2015) The 'sweet' spot of cellular pluripotency: protein glycosylation in human pluripotent stem cells and its applications in regenerative medicine. (PubMed: 25736263)
- PLoS One (2015) Increased risk of genetic and epigenetic instability in human embryonic stem cells associated with specific culture conditions. (PubMed: 25714340)
- Stem Cells Transl Med (2015) Enabling consistency in pluripotent stem cell-derived products for research and development and clinical applications through material standards. (PubMed: 25650438)
- Mov Disord (2015) Stem cell reprogramming: basic implications and future perspective for movement disorders. (PubMed: 25546831)
- Proc Natl Acad Sci U S A (2015) HDAC inhibition imparts beneficial transgenerational effects in Huntington's disease mice via altered DNA and histone methylation. (PubMed: 25535382)
- Stem Cell Reports (2015) hESC Differentiation toward an Autonomic Neuronal Cell Fate Depends on Distinct Cues from the Co-Patterning Vasculature. (PubMed: 26004631)
- Methods Mol Biol (2015) Generation of Induced Pluripotent Stem Cells from Mammalian Endangered Species. (PubMed: 26621593)
- Sci Rep (2015) Glycosyltransferase ST6GAL1 contributes to the regulation of pluripotency in human pluripotent stem cells. (PubMed: 26304831)
- Epigenomics (2015) DNA methylation fingerprint of neuroblastoma reveals new biological and clinical insights. (PubMed: 26067621)
- Stem Cell Reports (2014) Human neural precursor cells promote neurologic recovery in a viral model of multiple sclerosis. (PubMed: 24936469)
- Nat Commun (2014) Role of astroglia in Down's syndrome revealed by patient-derived human-induced pluripotent stem cells. (PubMed: 25034944)
- Nature (2014) Network biology: A compass for stem-cell differentiation. (PubMed: 25254472)
- Expert Rev Neurother (2014) Promoting remyelination: utilizing a viral model of demyelination to assess cell-based therapies. (PubMed: 25245576)
- Curr Protoc Stem Cell Biol (2014) Generation, Expansion, and Differentiation of Human Induced Pluripotent Stem Cells (hiPSCs) Derived From the Umbilical Cords of Newborns. (PubMed: 24838913)
- Genomics (2014) Application of a low cost array-based technique - TAB-Array - for quantifying and mapping both 5mC and 5hmC at single base resolution in human pluripotent stem cells. (PubMed: 25179373)
- Ann Neurol (2014) Epigenetic therapy for Friedreich ataxia. (PubMed: 25159818)
- Stem Cell Res Ther (2014) Neural stem cells genetically-modified to express neprilysin reduce pathology in Alzheimer transgenic models. (PubMed: 25022790)
- Circ Res (2014) Epigenetic regulation of pluripotency and differentiation. (PubMed: 24989490)
- Science (2014) Research capacity. Enabling the genomic revolution in Africa. (PubMed: 24948725)