Characterization and applications of human blastoids for understanding early human embryogenesis
Grant Award Details
Grant Type:
Grant Number:
DISC0-14503
Investigator(s):
Disease Focus:
Human Stem Cell Use:
Award Value:
$1,402,137
Status:
Active
Grant Application Details
Application Title:
Characterization and applications of human blastoids for understanding early human embryogenesis
Public Abstract:
Research Objective
Our work will yield an improved stem-cell based embryo model that we will explore with various omics approaches and genetic screens to gain insights into the pathways that control human embryos.
Impact
An improved stem cell-based embryo model is a crucial step for in-depth studies of human development and will enhance our ability to understand and treat infertility and screen for new contraceptives.
Major Proposed Activities
Our work will yield an improved stem-cell based embryo model that we will explore with various omics approaches and genetic screens to gain insights into the pathways that control human embryos.
Impact
An improved stem cell-based embryo model is a crucial step for in-depth studies of human development and will enhance our ability to understand and treat infertility and screen for new contraceptives.
Major Proposed Activities
- To identify a method that enables robust formation of a stem cell-derived embryo model across diverse genetic backgrounds.
- Assess the transcriptional, epigenetic and karyotype state of the embryo model.
- Examine the paracrine signals and metabolomic regulation of the stem cell-derived embryo model.
- Perform whole-genome and high-content genetic screens and in-depth dissection of gene-specific knockouts with the stem cell-derived embryo model.
Statement of Benefit to California:
In 2014, 21,018 IVF procedures were performed in CA, resulting in 7,230 live-birth deliveries and 8,793 infants born. This shows that IVF is inefficient, causing providers to transfer multiple embryos, which can result in multiple births and adverse health effects. Our work has the potential to develop an improved embryo model for studies of human development, infertility, and IVF approaches. The project will also employ six individuals to carry out the project, creating jobs within the state.
