Human pluripotent stem cell-based therapeutics for preeclampsia
Preeclampsia (PE) is a pregnancy complication, characterized by high blood pressure and abnormal kidney function, which affects 5-8% of all pregnancies. It is responsible for a significant proportion of maternal…
Correlated time-lapse imaging and single cell molecular analysis of human embryo development
We understand little about human development especially at the earliest stages. Yet human developmental biology is very important to stem cell biology and regenerative medicine for two reasons: 1) Understanding…
Molecular Mechanisms of Trophoblast Stem Cell Specification and Self-Renewal
Prematurity/preterm birth is the leading cause of neonatal death in the U.S. and in California. During an average day in California, 149 babies are born preterm. These babies are at…
Derivation and comparative analysis of human pluripotent ESCs, iPSCs and SSCs: Convergence to an embryonic phenotype
This is an unprecedented time in stem cell biology and regenerative medicine. Today, we have cell lines and tools that did not exist just a few years ago. Indeed, human…
Trophoblast differentiation of human ES cells.
Human embryonic stem (ES) cells have the potential to form any cell type, but ironically, the first cell lineage to form during development still represents a surprising challenge. The first…
Human Embryonic Stem Cell Differentiation to Trophoblast: Basic Biology and Clinical Translation to Improve Human Fertility
In addition to the important potential applications for transplantation and treatment of chronic diseases, human embryonic stem cells (hESC) are also a valuable resource to study early human development relevant…
Maturation of Human Oocytes for SCNT and Embryonic Stem Cell Derivation
A major issue for tissue and cell therapy in regenerative medicine is the immune rejection of grafts originated from a non-compatible individual. Mature eggs contain factors essential for the re-programming…
High-Throughput Discovery of Embryo Formation Factors Using Stem Cell-Based Human Embryo Models
Research Objective To integrate emulsion microfluidics-based encapsulation with AI-powered imaging to develop a high-throughput pipeline for stem cell-based embryo models. Impact We will overcome key bottlenecks to drive breakthroughs in…