Upregulation of CD11A on Hematopoietic Stem Cells Denotes the Loss of Long-Term Reconstitution Potential.

Journal: 
Stem Cell Reports
Publication Year: 
2014
Authors: 
John W Fathman
Nathaniel B Fernhoff
Jun Seita
Connie Chao
Vanessa M Scarfone
Irving L Weissman
Matthew A Inlay
PubMed link: 
25418718
Public Summary: 
Small numbers of hematopoietic stem cells (HSCs) generate large numbers of mature effector cells through the successive amplification of transiently proliferating progenitor cells. HSCs and their downstream progenitors have been extensively characterized based on their cell-surface phenotype and functional activities during transplantation assays. These cells dynamically lose and acquire specific sets of surface markers during differentiation, leading to the identification of markers that allow for more refined separation of HSCs from early hematopoietic progenitors. Here, we describe a marker, CD11A, which allows for the enhanced purification of mouse HSCs. We show through in vivo transplantations that upregulation of CD11A on HSCs denotes the loss of their long-term reconstitution potential. Surprisingly, nearly half of phenotypic HSCs (defined as Lin(?)KIT(+)SCA-1(+)CD150(+)CD34(?)) are CD11A(+) and lack long-term self-renewal potential. We propose that CD11A(+)Lin(?)KIT(+)SCA-1(+)CD150(+)CD34(?) cells are multipotent progenitors and CD11A(?)Lin(?)KIT(+)SCA-1(+)CD150(+)CD34(?) cells are true HSCs.
Scientific Abstract: 
Small numbers of hematopoietic stem cells (HSCs) generate large numbers of mature effector cells through the successive amplification of transiently proliferating progenitor cells. HSCs and their downstream progenitors have been extensively characterized based on their cell-surface phenotype and functional activities during transplantation assays. These cells dynamically lose and acquire specific sets of surface markers during differentiation, leading to the identification of markers that allow for more refined separation of HSCs from early hematopoietic progenitors. Here, we describe a marker, CD11A, which allows for the enhanced purification of mouse HSCs. We show through in vivo transplantations that upregulation of CD11A on HSCs denotes the loss of their long-term reconstitution potential. Surprisingly, nearly half of phenotypic HSCs (defined as Lin(?)KIT(+)SCA-1(+)CD150(+)CD34(?)) are CD11A(+) and lack long-term self-renewal potential. We propose that CD11A(+)Lin(?)KIT(+)SCA-1(+)CD150(+)CD34(?) cells are multipotent progenitors and CD11A(?)Lin(?)KIT(+)SCA-1(+)CD150(+)CD34(?) cells are true HSCs.