Proteomics of the heart.
Publication Year:
2024
PubMed ID:
38300522
Funding Grants:
Public Summary:
Mass spectrometry-based proteomics is a powerful technique that helps scientists study proteins and how they change in the heart at a very detailed level. It shows which proteins are present, how they interact, and how they change in different conditions. This helps us understand heart diseases better and could lead to new treatments. Recent improvements allow researchers to study proteins in single cells and use robots to prepare samples more efficiently. Using animal models, scientists have learned a lot about heart problems. As this technology keeps improving, it will become more useful in hospitals, helping doctors and patients better understand and treat heart disease.
Scientific Abstract:
Mass spectrometry-based proteomics is a sophisticated identification tool specializing in portraying protein dynamics at a molecular level. Proteomics provides biologists with a snapshot of context-dependent protein and proteoform expression, structural conformations, dynamic turnover, and protein-protein interactions. Cardiac proteomics can offer a broader and deeper understanding of the molecular mechanisms that underscore cardiovascular disease, and it is foundational to the development of future therapeutic interventions. This review encapsulates the evolution, current technologies, and future perspectives of proteomic-based mass spectrometry as it applies to the study of the heart. Key technological advancements have allowed researchers to study proteomes at a single-cell level and employ robot-assisted automation systems for enhanced sample preparation techniques, and the increase in fidelity of the mass spectrometers has allowed for the unambiguous identification of numerous dynamic posttranslational modifications. Animal models of cardiovascular disease, ranging from early animal experiments to current sophisticated models of heart failure with preserved ejection fraction, have provided the tools to study a challenging organ in the laboratory. Further technological development will pave the way for the implementation of proteomics even closer within the clinical setting, allowing not only scientists but also patients to benefit from an understanding of protein interplay as it relates to cardiac disease physiology.