Floxin, a resource for genetically engineering mouse ESCs.

Journal: 
Nat Methods
Publication Year: 
2010
Authors: 
Veena Singla
Julie Hunkapiller
Nicole Santos
Allen D Seol
Andrew R Norman
Paul Wakenight
William C Skarnes
Jeremy F Reiter
PubMed link: 
19966808
Public Summary: 
We developed a novel method of genetically modifying mouse embryonic stem cells. This technology is being used for many purposes and by many labs.
Scientific Abstract: 
We describe a method for the highly efficient and precise targeted modification of gene trap loci in mouse embryonic stem cells (ESCs). Through the Floxin method, gene trap mutations were reverted and new DNA sequences inserted using Cre recombinase and a shuttle vector, pFloxin. Floxin technology is applicable to the existing collection of 24,149 compatible gene trap cell lines, which should enable high-throughput modification of many genes in mouse ESCs.