Public Summary of Scientific Progress: The central mission of our Berkeley CIRM Shared Stem Cell Facility (SSCF) is to provide our East Bay users with knowledge, expertise, training, and equipment to advance scientific knowledge of human embryonic, induced pluripotent, and adult stem cells. The Flow Cytometry Facility in 461 Li Ka Shing houses the CIRM funded cell sorter. The B129 Stanley suite contains cell culture hoods, four incubators, and other essential lab equipment. The SSCF also has an automated wide field ImageXpress Micro, and two analytical flow cytometers, and a 2-photon and confocal fluorescence microscope. In B203 Stanley, there is a BioRad BioPlex MAGPIX reader for magnetic bead–based multiplexed immunoassays. We have reached a steady state usage of ~90 students and postdoctoral fellows from the laboratories of 25 PIs. Based on the interests of the users, we have continued in Year 7NCE to maintain state of the art instrumentation for the research community by using some of Year6 funds to help bridge salary and instrument service contract funding from CIRM funding to alternatives. We are committed to continuing operating this facility, as it has become an important resource to the UC Berkeley research community.
The previously highlighted PIs continue to have success using SSCF instrumentation and we would like to highlight publications by researchers who have made significant advancements to their research.
Hojeong Jeon, Sangmo Koo, Willie Mae Reese, Peter Loskill, Costas P Grigoropoulos, Kevin E Healy. (2015) “Directing cell migration and organization via nanocrater-patterned cell-repellent interfaces.” Nature Materials 14(9): 918-923 (PMCID: PMC4545687).
Kevin M Tharp, Amit K Jha, Judith Kraiczy, Alexandra Yesian, Grigory Karateev, Riccardo Sinisi, Elena A Dubikovskaya, Kevin E Healy, Andreas Stahl. (2015) “Matrix-Assisted Transplantation of Functional Beige Adipose Tissue.” Diabetes 64(11): 3713-3724 (PMID: 26293504).
Peter Loskill, Sivan G Marcus, Anurag Mathur, Willie Mae Reese, Kevin E Healy. (2015) “μOrgano: A Lego®-Like Plug & Play System for Modular Multi-Organ-Chips.” PLoS One 10(10): e0139587 (PMID: 26440672).
Amit K Jha, Kevin M Tharp, Shane Browne, Jianqin Ye, Andreas Stahl, Yerem Yeghiazarians, Kevin E Healy. (2016) “Matrix metalloproteinase-13 mediated degradation of hyaluronic acid-based matrices orchestrates stem cell engraftment through vascular integration.” Biomaterials 89: 136-147 (PMID: 26967648).
Eda I Altiok, Jorge L Santiago-Ortiz, Felicia L Svedlund, Aline Zbinden, Amit K Jha, Deepika Bhatnagar, Peter Loskill, Wesley M Jackson, David V Schaffer, Kevin E Healy. (2016) “Multivalent hyaluronic acid bioconjugates improve sFlt-1 activity in vitro.” Biomaterials 93: 95-105 (PMID: 27086270).
Zheng. B., M.P. von See, E. Yu, B. Gunel, K. Lu, T. Vazin, D.V. Schaffer, P.W. Goodwill, and S.M. Conolly (2016) “Quantitative Magnetic Particle Imaging Monitors the Transplantation, Biodistribution, and Clearance of Stem Cells in Vivo.” Theranostics, 6:291-301 (PMID 26909106).
Luque, T., M.S. Kang, D.V. Schaffer, and S. Kumar (2016) “Microelastic Mapping of the Rat Dentate Gyrus.” Royal Society Open Science, 3:150702 (PMID 27152213).
Chen, S. Y.S. Na, M.E. Todhunger, S. Hsiao, A.W. Bremer, O.J. Scheideler, P.R. Bomdica, M.M. Haharbiz, Z.J. Gartner, D.V. Schaffer (2016) “Interrogating Stem Cell Fate Decisions with High-throughput Arrays of Cellular Communities.” Nature Communications, 7:10309 (PMID 26754526).
Zheng, V., T. Vazin, P.W. Goodwill, A. Conway, A. Verma, E. Ulku Saritas, D.V. Schaffer, and S.M. Conolly (2015) “Magnetic Particle Imaging Tracks the Long-term Fate of in Vivo Neural Cell Implants with High Image Contrast.” Scientific Reports, 5:14055 (PMID: 26358296).
Han, B.W., H. Layman, N.A. Rode, A. Conway, D.V. Schaffer, N.J. Boudreau, W.M. Jackson, and K.E. Healy (2015) “Multivalent Conjugates of Sonic Hedgehog Accelerate Diabetic Wound Healing.” Tissue Engineering, 21:2366-2378 (PMID 26154888).
Sia J, Sun R, Chu J, Li S. (2016) “Dynamic culture improves cell reprogramming efficiency.” Biomaterials 92:36-45 (PMID: 27031931).
Lee K, Rafi M, Feng X, Tang R, Lingampalli N, Aran K, Murthy N. (2015) “In vivo delivery of transcription factors with multifunctional oligonucleotides.” Nature Materials 14(7), 701-706 (PMID: 25915034).
Lee K, Yu P, Lingampalli N, Tang R, Kim H, Murthy N. (2015) “Peptide-enhanced mRNA transfection in cultured mouse cardiac fibroblasts and direct reprogramming towards cardiomyocyte-like cells.” Int J Nanomedicine 10, 1841-1854 (PMID: 25834424).
Kunwoo Lee, Michael Conboy, Fuguo Jiang, Hyun Jin Kim, Mark A. Dewitt, Vanessa A. Mackley, Wen-chin Huang, Kevin Chang, Anirudh Rao, Colin Skinner, Tamanna Shobha, Freeman Lan, Nicolas L. Bray, Song Li, Jacob E. Corn, Kazunori Kataoka, Jennifer A. Doudna, Irina Conboy, Niren Murthy “Gold nanoparticle-mediated in vivo delivery of CRISPR/Cas9 ribonucleoprotein and donor DNA.” (Submitted).