The UC Davis Translational Human Embryonic Stem Cell Shared Research Facility (TSRF) provides a resource for California investigators and trainees to study human embryonic stem cells and related stem and progenitor cell populations in a highly collaborative environment. The TSRF supports research for investigators and provides training and guidance to those new to human embryonic stem cell research or those in need of additional guidance with the culture and study of pluripotent stem cells. The TSRF includes laboratories for cell culture, flow cytometry and cell sorting, techniques for cellular and molecular analysis of the cells, and essential services including assistance with cryopreservation and storage of cell banks for experiments. Calendar sign-up is available for use of the laboratories and equipment within the facility. Examples of services include high quality mouse embryonic feeders (MEFs) on a recharge basis and screened reagents for investigator use. Trainees from CIRM training programs receive training in cell culture and related techniques, and are able to use the facility to pursue their research projects under the guidance of experienced faculty and staff.