Year 3
During the last year of support, we employed the knock-in hESCs in which the EGFP gene was introduced into the human hematopoietic stem cell (HSC)-specific locus. Together with CD34 marker, we purified and gene profiled the hESC-derived CD34+GFP+ cells. By establising the humanized mouse model with functional human immune system that can reject the cardiomyocytes from another individual, we evaluated the feasibility to induce immune tolerance of hESC-derived allografts with hESC-derived hematopoietic cells.