Year 2

Understanding the mechanisms underlying cell fate decision of human embryonic stem cells (hESCs) is critical for materializing the therapeutic potential of hESCs. However, quantitative delineation of the combinatorial effects of regulators and computational modeling with sufficient molecular details of their roles during hESC differentiation are largely lacked. In the reporting period, we have systematically identified lineage-specific regulators, computationally predicted genetic perturbations of these regulators that can direct hESC differentiate to a specific lineage.