Year 1

The goal of the work is to develop a tool to predict which IPS cells will efficiently produced keratinocytes by precisely defining the chromatin dynamics during differentiation, paying particular attention to the contributions from morphogens and those from the major lineage selectors. We find enormous chromatin accessibility changes that are lineage-selector independent, but morphogen-dependent. We also find that the binding sites for the major lineage-selector TP63 are pre-determined in H9 cells, allowing TP63 to edit the morphogen-induced epigenetic landscape. These data provide the basis for a simple tool to begin predicting cellular response and rationale in-process manufacturing assays for clinical production.