Year 1

The goal of this study is to identify mutations in human iPSC lines and determine their likely origin. To accomplish this we previously used mouse models to develop and validate new methods to isolate iPSC lines from the same precursor donor cell and to perform comprehensive whole genome sequencing based mutation detection . In this grant period we have translated our iPSC tracing and lineage analysis methods to human iPSCs, established two methods to produce iPSCs using non integrating methods and improved our bioinformatic detection of mutations. We are now poised carry out the remainder of the proposed work within the timeframe allotted.