Year 1

We have made significant progress in both aims proposed in the application during the first year of support. We generated the targeting construct to introduce a florescent marker into the human hematopoietic stem cell (HSC)-specific locus to identify and purify hESC-derived HSCs. We establish the conditions to differentiate hESCs into HSCs. In addition, we establish a humanized mice with functional human immune system that can effective reject cells derived from another individual.