Promoting survival and countering hypertrophy of pluripotent stem cell (PSC)-derived chondrocytes

Funding Type: 
Basic Biology V
Grant Number: 
RB5-07230
Investigator: 
Award Value: 
$1,146,468
Disease Focus: 
Arthritis
Stem Cell Use: 
Embryonic Stem Cell
Status: 
Active
Public Abstract: 

Degenerative joint disease, also known as osteoarthritis, currently affects more than 20 million people in the USA alone, making articular cartilage restoration one of the major priorities in medicine. Articular chondrocyte progenitors are likely to be present only early in development, which explains why previous attempts to engineer articular cartilage using adult stem cells have been unsuccessful. Recapitulation of the human chondrogenic program using human pluripotent stem cells (hPSC) may represent a groundbreaking system for cartilage restoration. We hypothesize that regeneration of articular cartilage requires not only generation of structural cartilage cells, but also a supportive “niche” component absent or not fully represented in the adult joint. The overall goal of this research proposal is to dissect the cellular and molecular components of this chondrogenic “niche” essential for the survival, maintenance and expansion of cartilage cells produced from hPSC. The findings from these studies will not only contribute towards our understanding of how articular cartilage is formed during hPSC differentiation, but will also have broad applicability for the production of other types of tissues from hPSC. Most importantly, the ability to control differentiation from hPSC into the articular chondrocyte lineage may provide an unlimited source of matched cells for transplantation in patients with joint cartilage degeneration

Statement of Benefit to California: 

The unique combination of pluripotentiality and an unlimited capacity for proliferation have raised the hope that pluripotent stem cells (PSC) will one day provide an inexhaustible source of tissue for transplantation and regeneration. Degenerative joint cartilage disease, also known as osteoarthritis, is one of the most common chronic diseases among Californians, causing significant pain and loss of mobility and impairing earning capacity. Tens of thousands of Californians go through invasive and expensive total joint replacement surgery every year. This proposal explores the question of what unique molecular signals control the survival and maintenance of cartilage cells (also known as chondrocytes) produced during the differentiation of PSC. The research proposed in this application has broad potential benefits for Californians, both through the biological questions it will answer and the relevance of its findings for clinical translation. The development of a human cell culture system that could expand the number of available articular chondrocytes would provide new opportunities for transplantation for patients with cartilage injury or degenerative arthritis and potentially delay or even prevent these patients from needing joint replacement procedures. All the scientific findings and technical tools developed in this proposal will be made available to researchers throughout California, according to the guidelines of the California Institute of Regenerative Medicine

Progress Report: 

Degenerative joint disease or osteoarthritis (OA) remains one of the major problems in modern medicine. One of the key pathological features of OA is death of cartilage forming cells - chondrocytes. Unfortunately chondrocytes have minimal regenerative capacity. Generation of functional chondrocytes from human embryonic stem cells may represent novel approach for cartilage repair. Recent tests in our laboratory have shown the embryonic stem cell (ESC)-derived cartilage cells are similar to cartilage cells in the joints. However, these cells are proliferative and functional only in the presence of specific factors forming so-called cartilage cell niche. Unfortunately this niche becomes dysfunctional with aging making survival of transplanted ESC-derived chondrocytes nearly impossible. We have now defined the most important molecular pathways responsible for the maintenance of the most primitive cartilage cells. Co-transplantation of the ECS-derived chondrocytes and these molecules is expected to yield the most efficient functional outcomes in patients with arthritis. This work may provide new transplantation opportunities for patients with cartilage injury or arthritis and delay or even prevent these patients from needing joint replacement procedures. In addition to detailed basic studies the work in our lab is now focused on generating ESC-derived chondrocytes on a large scale and testing their ability to repair cartilage injuries.