Grant Award Details

Optimizing self-renewal signaling kinetics to stabilize ex vivo hematopoietic stem cell expansion
Grant Number: 
DISC1-10555
Project Objective: 
  • The objective is to develop stable, ex vivo culture conditions for mouse and human hematopoietic stem cells by modulating TPO signaling

Investigator: 
Institution: 
Type: 
PI
Disease Focus: 
Blood Disorders
Human Stem Cell Use: 
Adult Stem Cell
Award Value: 
$210,906
Status: 
Active

Grant Application Details

Application Title: 
  • Optimizing self-renewal signaling kinetics to stabilize ex vivo hematopoietic stem cell expansion
Public Abstract: 

Research Objective

We aim to develop conditions for stable expansion of blood stem cells outside of the body

Impact

Blood stem cells are a rare but necessary cell type for curative bone marrow transplantation and related gene therapies. Stable blood stem cell expansion will increase therapy availability and success

Major Proposed Activities

  • Validate a fully defined all-recombinant protein culture system for long-term HSC expansion
  • Develop pharmacological strategies to provide robust ex vivo human HSC maintenance and expansion
Statement of Benefit to California: 

Blood stem cell availability is a major bottleneck in bone marrow transplantation, a curative therapy for numerous blood diseases. Blood stem cells currently cannot be stably maintained outside the body. Stable culture conditions would therefore increase blood stem cell availability, and improve accessibility to clinical bone marrow transplantation and related gene therapies. This research will ultimately improve bone marrow transplantation and related gene therapies for patients in California.