Functional Genomic Analysis of Chemically Defined Human Embryonic Stem Cells
Grant Award Details
Human Stem Cell Use:
Cell Line Generation:
Grant Application Details
Functional Genomic Analysis of Chemically Defined Human Embryonic Stem Cell
Regenerative medicine holds the promise that tissues can be engineered in vitro and then transplanted into patients to treat debilitating diseases. Human Embryonic Stem Cells differentiate into a wide array of adult tissue types and are thought to be the best hope for future regenerative therapies. This grant has three main goals: 1. The creation of new human embryonic stem cells in animal free conditions which will allow for future therapeutic uses. 2. The creation of human embryonic stem cell that contain mutations in their genomes that cause diseases, including cystic fibrosis, muscular dystrophy, Downs Syndrome and many others. These lines can be used to study these diseases and to test potential therapies 3. A close biological assessment of one of the first tissues to arise during differentiation of human embryonic stem cells – the endoderm. Since the endoderm eventually, during many days of development, becomes the pancreas, liver, and gut. It is critically important that we know everything about this very specialized tissue if we are to attempt to engineer these organs in the laboratory. Our overwhelming goal is to provide tools that clinician can use to treat disease whether it is to establish new and improved human embryonic stem cell lines or to find new ways of creating endodermal tissues within the laboratory for future therapeutic uses.
Statement of Benefit to California:
This grant will provide to the state of California new human embryonic stem cell lines that could be used in future therapeutic uses. It will also provide disease specific human embryonic stem cell lines that can be used to study disease and as models to test pharmacological compounds to treat disease. We will also provide a characterization of tissues generated from the new human embryonic stem cells which we hope will someday aid in the formation of liver, lung and pancreas.
- Dev Biol (2011): Chromatin and transcriptional signatures for Nodal signaling during endoderm formation in hESCs. (PubMed: 21741376)
- Genome Res (2009): Distinct DNA methylation patterns characterize differentiated human embryonic stem cells and developing human fetal liver. (PubMed: 19273619)
- Stem Cells (2011): Distinguishing Human Cell Types Based on Housekeeping Gene Signatures. (PubMed: 22162332)
- Genes Dev (2011): HEB and E2A function as SMAD/FOXH1 cofactors. (PubMed: 21828274)
- Stem Cells (2014): JMJD5 regulates cell cycle and pluripotency in human embryonic stem cells. (PubMed: 24740926)
- PLoS One (2011): A New FACS Approach Isolates hESC Derived Endoderm Using Transcription Factors. (PubMed: 21408072)