We just published a paper supported by CIRM funding showing that knocking out c- and N-myc in mESC leads to a wave of differentiation-associated gene expression, decreased cell cycling, and a moderate elevation of apoptosis. The myc-deficient mESC also fail to contribute to early embryogenesis. This is the first analysis of a role for myc genes in early embryogenesis.
We think that in part that Myc contributes to iPS formation by repressing differentiation-associated gene expression (ala Sridharan, et al).
So to induce pluripotency Myc appears to be doing what much the same job as it does to maintain pluripotency in ESC. A role in cell cycle is also involved.
Cancer genes also involved in embryogenesis, stem cell maintenance
Posted: June 14, 2010