Synthetic surfaces for human embryonic stem cell culture.

Journal: 
J Biotechnol
Publication Year: 
2010
Authors: 
Poornima Kolhar
Venkata Ramana Kotamraju
Sherry T Hikita
Dennis O Clegg
Erkki Ruoslahti
PubMed link: 
20132848
Public Summary: 
Human embryonic stem cells (hESCs) have numerous potential biomedical applications owing to their unique abilities for self-renewal and pluripotency. Successful clinical application of hESCs and derivatives necessitates the culture of these cells in a fully defined environment. We have developed a novel peptide-based surface that uses a high-affinity cyclic RGD peptide for culture of hESCs under chemically defined conditions.
Scientific Abstract: 
Human embryonic stem cells (hESCs) have numerous potential biomedical applications owing to their unique abilities for self-renewal and pluripotency. Successful clinical application of hESCs and derivatives necessitates the culture of these cells in a fully defined environment. We have developed a novel peptide-based surface that uses a high-affinity cyclic RGD peptide for culture of hESCs under chemically defined conditions.