In this study, we investigated the spatial variation of vitreous oxygen consumption in enucleated porcine eyes. We fabricated a custom oxygen source, implanted it in the mid vitreous and the posterior vitreous and found that the oxygen concentration profiles were statistically different between these two locations; suggesting a spatial variation in vitreous oxygen consumption. In conjunction with that finding, we observed statistically different concentrations of ascorbate across the two locations and quantified the reaction rate between ascorbate and oxygen invitreous