Multiscale, multispectral fluorescence lifetime imaging using a double-clad fiber.

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Publication Year:

2019

Authors:

Benjamin E Sherlock, Cai Li, Xiangnan Zhou, Alba Alfonso-Garcia, Julien Bec, Diego Yankelevich, Laura Marcu

PubMed ID:

31042209

Funding Grants:

Multimodal platform combining optical and ultrasonic technologies for in vivo nondestructive evaluation of engineered vascular tissue constructs

Public Summary:

We developed a single-fiber optic imaging system based on a custom double-clad fiber to acquire multispectral fluorescence lifetime images at two different spatial resolutions that will allow switching imaging scales with enhanced flexibility.

Scientific Abstract:

Fiber-based imaging of tissue autofluorescence using ultraviolet (UV) excitation is a highly flexible tool used to probe structure and composition. In this Letter, we report, to the best of our knowledge, the first results from a single-fiber imaging system employing a custom double-clad fiber to acquire multispectral fluorescence lifetime images at two distinct spatial resolutions. We characterize the lateral point spread function and fluorescent background of the system and show how enhanced resolution can identify features such as trabeculae in ex vivo murine bone samples.