Since hippocampal DG neurons have been thought to be involved in Schizophrenia (SCZD), we applied our protocol to SCZD patient-derived stem cells to see if we can uncover important clues as to the roles that these cells may play in the disease. We found that DG neurons generated from SCZD patient-derived stem cells exhibited significantly lower levels of key proteins that are crucial for neuronal development , resulting in reduced neuronal activity, and potentially causing the cognitive deficits clinically observed in these patients. Our approach offers important insights into the neurodevelopmental aspects of SCZD and may be a promising tool for drug screening and personalized medicine.