From MEFs to Matrigel 3: passaging hESCs from Matrigel onto Matrigel.

Publication Year:

2008

Authors:

Jin Zhang, Ivan Khvorostov, Michael Teitell

PubMed ID:

19066542

Funding Grants:

Role of Mitochondria in Self-Renewal Versus Differentiation of Human Embryonic Stem Cells

Public Summary:

This video demonstrates how to maintain the growth of human embryonic stem cells (hESCs) in feeder cell-free conditions and how to continuously passage hESCs in feeder cell-free conditions. Confirmation of hESC pluripotency grown in feeder cell-free conditions by immunofluorescence microscopy is also demonstrated.

Scientific Abstract:

This video demonstrates how to maintain the growth of human embryonic stem cells (hESCs) in feeder cell-free conditions and how to continuously passage hESCs in feeder cell-free conditions. Confirmation of hESC pluripotency grown in feeder cell-free conditions by immunofluorescence microscopy is also demonstrated.