From MEFs to Matrigel 2: splitting hESCs from MEFs onto Matrigel.
Journal:
J Vis Exp
Publication Year:
2008
PubMed link:
19066543
Funding Grants:
Public Summary:
This video demonstrates how to grow human embryonic stem cells (hESCs) on mouse embryonic fibroblast (MEF) feeder cells, how to passage hESCs from MEF plates to feeder cell-free Matrigel plates.
Scientific Abstract:
This video demonstrates how to grow human embryonic stem cells (hESCs) on mouse embryonic fibroblast (MEF) feeder cells, how to passage hESCs from MEF plates to feeder cell-free Matrigel plates.