Long-term repair of porcine articular cartilage using cryopreservable, clinically compatible human embryonic stem cell-derived chondrocytes.

NPJ Regen Med
Publication Year: 
Frank A Petrigliano
Nancy Q Liu
Siyoung Lee
Jade Tassey
Arijita Sarkar
Yucheng Lin
Liangliang Li
Yifan Yu
Dawei Geng
Jiankang Zhang
Ruzanna Shkhyan
Jacob Bogdanov
Ben Van Handel
Gabriel B Ferguson
Youngjoo Lee
Svenja Hinderer
Kuo-Chang Tseng
Aaron Kavanaugh
J Gage Crump
April D Pyle
Katja Schenke-Layland
Fabrizio Billi
Liming Wang
Jay Lieberman
Mark Hurtig
Denis Evseenko
PubMed link: 
Public Summary: 
This publication shows how human embryonic stem cell-derived cartilage can be used to reconstruct skeletal joints in pigs.
Scientific Abstract: 
Osteoarthritis (OA) impacts hundreds of millions of people worldwide, with those affected incurring significant physical and financial burdens. Injuries such as focal defects to the articular surface are a major contributing risk factor for the development of OA. Current cartilage repair strategies are moderately effective at reducing pain but often replace damaged tissue with biomechanically inferior fibrocartilage. Here we describe the development, transcriptomic ontogenetic characterization and quality assessment at the single cell level, as well as the scaled manufacturing of an allogeneic human pluripotent stem cell-derived articular chondrocyte formulation that exhibits long-term functional repair of porcine articular cartilage. These results define a new potential clinical paradigm for articular cartilage repair and mitigation of the associated risk of OA.