Light-Patterned RNA Interference of 3D-Cultured Human Embryonic Stem Cells.

Journal: 
Adv Mater
Publication Year: 
2016
Authors: 
Xiao Huang
Qirui Hu
Yifan Lai
Demosthenes P Morales
Dennis O Clegg
Norbert O Reich
PubMed link: 
27787919
Public Summary: 
n/a
Scientific Abstract: 
A new method of spatially controlled gene regulation in 3D-cultured human embryonic stem cells is developed using hollow gold nanoshells (HGNs) and near-infrared (NIR) light. Targeted cell(s) are discriminated from neighboring cell(s) by focusing NIR light emitted from a two-photon microscope. Irradiation of cells that have internalized HGNs releases surface attached siRNAs and leads to concomitant gene downregulation.