Light-Patterned RNA Interference of 3D-Cultured Human Embryonic Stem Cells.

Journal: 
Adv Mater
Publication Year: 
2016
Authors: 
Xiao Huang
Qirui Hu
Yifan Lai
Demosthenes P Morales
Dennis O Clegg
Norbert O Reich
PubMed link: 
27787919
Funding Grants: 
Stem cell based treatment strategy for Age-related Macular Degeneration (AMD)
Phase 1 Safety Assessment of CPCB-RPE1, hESC-derived RPE Cell Coated Parylene Membrane Implants, in Patients with Advanced Dry Age Related Macular Degeneration
Public Summary: 
A new method of spatially controlled gene regulation in 3D-cultured human embryonic stem cells is developed using hollow gold nanoshells (HGNs) and near-infrared (NIR) light. Targeted cell(s) are discriminated from neighboring cell(s) by focusing NIR light emitted from a two-photon microscope. Irradiation of cells that have internalized HGNs releases surface attached siRNAs and leads to concomitant gene downregulation.
Scientific Abstract: 
A new method of spatially controlled gene regulation in 3D-cultured human embryonic stem cells is developed using hollow gold nanoshells (HGNs) and near-infrared (NIR) light. Targeted cell(s) are discriminated from neighboring cell(s) by focusing NIR light emitted from a two-photon microscope. Irradiation of cells that have internalized HGNs releases surface attached siRNAs and leads to concomitant gene downregulation.
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