Library-free methylation sequencing with bisulfite padlock probes.

Nat Methods
Publication Year: 
D Diep
N Plongthongkum
A Gore
H L Fung
R Shoemaker
K Zhang
PubMed link: 
Public Summary: 
Scientific Abstract: 
Targeted quantification of DNA methylation allows for interrogation of the most informative loci across many samples quickly and cost-effectively. Here we report improved bisulfite padlock probes (BSPPs) with a design algorithm to generate efficient padlock probes, a library-free protocol that dramatically reduces sample-preparation cost and time and is compatible with automation, and an efficient bioinformatics pipeline to accurately obtain both methylation levels and genotypes from sequencing of bisulfite-converted DNA.