Induced pluripotent stem cells and embryonic stem cells are distinguished by gene expression signatures.

Cell Stem Cell
Publication Year: 
Mark H Chin
Mike J Mason
Wei Xie
Stefano Volinia
Mike Singer
Cory Peterson
Gayane Ambartsumyan
Otaren Aimiuwu
Laura Richter
Jin Zhang
Ivan Khvorostov
Vanessa Ott
Michael Grunstein
Neta Lavon
Nissim Benvenisty
Carlo M Croce
Amander T Clark
Tim Baxter
April D Pyle
Mike A Teitell
Matteo Pelegrini
Kathrin Plath
William E Lowry
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Public Summary: 
Scientific Abstract: 
Induced pluripotent stem cells (iPSCs) outwardly appear to be indistinguishable from embryonic stem cells (ESCs). A study of gene expression profiles of mouse and human ESCs and iPSCs suggests that, while iPSCs are quite similar to their embryonic counterparts, a recurrent gene expression signature appears in iPSCs regardless of their origin or the method by which they were generated. Upon extended culture, hiPSCs adopt a gene expression profile more similar to hESCs; however, they still retain a gene expression signature unique from hESCs that extends to miRNA expression. Genome-wide data suggested that the iPSC signature gene expression differences are due to differential promoter binding by the reprogramming factors. High-resolution array profiling demonstrated that there is no common specific subkaryotypic alteration that is required for reprogramming and that reprogramming does not lead to genomic instability. Together, these data suggest that iPSCs should be considered a unique subtype of pluripotent cell.