Generation of Induced Pluripotent Stem Cells from Mammalian Endangered Species.

Journal: 
Methods Mol Biol
Publication Year: 
2015
Authors: 
Inbar Friedrich Ben-Nun
Susanne C Montague
Marlys L Houck
Oliver Ryder
Jeanne F Loring
PubMed link: 
26621593
Public Summary: 
For some highly endangered species there are too few reproductively capable animals to maintain adequate genetic diversity, and extraordinary measures are necessary to prevent their extinction. Cellular reprogramming is a means to capture the genomes of individual animals as induced pluripotent stem cells (iPSCs), which may eventually facilitate reintroduction of genetic material into breeding populations. Here, we describe a method for generating iPSCs from fibroblasts of mammalian endangered species.
Scientific Abstract: 
For some highly endangered species there are too few reproductively capable animals to maintain adequate genetic diversity, and extraordinary measures are necessary to prevent their extinction. Cellular reprogramming is a means to capture the genomes of individual animals as induced pluripotent stem cells (iPSCs), which may eventually facilitate reintroduction of genetic material into breeding populations. Here, we describe a method for generating iPSCs from fibroblasts of mammalian endangered species.