The development of better methods to carry genes into stem cells would enable a number of applications, including investigating the role of key genes in the behavior of the stem cell, delivery of specific genes to control stem cell proliferation or differentiation, delivery factors to enhance the therapeutic efficacy of stem cells and their progeny, and genetically modifying the cells to serve as models of human disease. Gene delivery vehicles based on adeno-associated virus, i.e. AAV vectors, have demonstrated the capacity for efficient delivery to numerous cells, but their application to stem cells has been limited by low delivery efficiency. Due to their considerable advantages, however, engineering AAV delivery systems to enhance gene delivery to stem cells may have an impact in stem cell biology and therapy. Therefore, we have used a high throughput protein engineering technique to improve the efficiency of AAV gene delivery to neural stem cells (NSCs). A novel AAV variant, carrying an insertion of a selected peptide sequence on the viral surface, emerged from this approach. Importantly, this evolved AAV variant mediated efficient gene delivery to rat, mouse, and human NSCs, as well as the capacity to edit endogenous genes within adult NSCs, and it is thus promising for applications ranging from basic stem cell biology to clinical translation.