Current method of cell culture often uses single proteins and material which do not represent the native environment where the cells normally reside in the tissue. We are interested in reproducing the native environment by extracting the extracellular matrix, which is composed of different types of protein surround the cells, from the brain. We developed a method which allowed us to remove the cellular components and preserve the extracellular matrix from pig brains. When we cultured neurons derived from induced pluripotent stem cells developed from patients, we found that the decellularized pig matrix is superior in promoting maturation of neuronal morphology compared to standard coating. Our work demonstrates the ability to use decellularized brain extracellular matrix for cell culture and tissue engineering applications.