This study describes a technique for leveraging a system of mesenchymal stem cellmediated, mAb-assisted bone regeneration. Here we describe a bone regeneration strategy based on in vitro and in vivo experiments which demonstrate the capacity of hBMMSCs (human bone marrow-multipotent stromal cells) to respond to the inductive signals provided by an anti-BMP2 mAb. We utilized the ability of anti-BMP2 mAb to trap and tether endogenous BMP2 (Bone morphogenetic protein 2) ligands for the directed osteodifferentiation of hBMMSCs using an RGD-alginate microencapsulation system. The advantages of this system include its simplicity and ease with which it can be modified to encapsulate hBMMSCs in an injectable and biodegradable alginate hydrogel, yielding a 3- dimensional, cell delivery scaffold for bone tissue engineering.