In this work, we show that human iPSC-derived neurons cultivated in the presence of astrocytes mature faster than neurons without. We performed several morphological and electrophsyoloigcal experiments to prove that the presence of astrocytes are essential for neurons to form connections (synapses) and communicate with each other. In conclusion, we demonstrated the positive effects of astrocytes and showed a new protocol to speed up neuronal maturation. This would likely be useful for follow up experiments on disease modeling and drug screening.