Application of a low cost array-based technique – TAB-Array – for quantifying and mapping both 5mC and 5hmC at single base resolution in human pluripotent stem cells.

We have developed a method for mapping 5-hydroxymethylcytosine (5hmC) in human pluripotent stem cells and their derivatives. We find that 5hmC profiling reveals far more about epigenetic changes during differentiation of human pluripotent stem cells than conventional 5-methylcytosine profiling.