Antibody screening data of human midgestation liver cells with a focus on hematopoietic, liver sinusoidal endothelial, and hepatoblast cell-populations.

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Public Summary:
This manuscript describes a large-scale screening of antigens expressed by the different cell populations that reside in the prenatal human liver at midgestation. A standard set of markers was used in the screen to allow for identification of blood cells, hepatoblasts (early liver-cell progenitors), and liver sinusoidal endothelial cells. Using flow cytometry, we screened an additional 332 antigens in addition to these standard markers to determine the expression of these antigens by different cell populations. The manuscript describes a basic analysis of the data that has been performed and made available on a public database. The entire raw dataset has been made publicly available so that researchers interested in studying the developmental biology of the human liver can have access to these data for their own work.
Scientific Abstract:
OBJECTIVES: Cell-surface antigen screening was performed on human fetal liver cells using flow cytometry. The goal was to provide proteomic expression data on a number of human fetal liver cell populations that can inform studies on developmental hepatology and hematology. DATA DESCRIPTION: A 21 weeks' gestation liver was depleted of erythrocytes prior to antibody staining. Screening was performed using phycoerythrin-labelled antibodies against 332 antigens. In addition to these antibodies, all samples were stained for CD14, CD45, CD235a, and CD326 (epithelial cell adhesion molecule - EpCAM). Subpopulations of fetal liver cells were identified using the co-stained antigens. Hematopoietic cells were identified by their expression of CD45 and CD235a; non-hematopoietic cells were further subdivided based on CD14 and CD326 expression. CD326(++)CD14(low) hepatoblasts and CD14(++) liver sinusoidal endothelial cells were analyzed for the frequency and intensity of antigen expression. Analyzed flow cytometry data are presented for the expression of the antigens on hematopoietic cells and on non-hematopoietic cells in the context of CD14 and CD326 expression.