More than 1800 gene therapy clinical trials worldwide have targeted a wide range of conditions including cancer, heart diseases, and genetic diseases. Biological (i.e. viral), chemical, and physical approaches have been developed to deliver genes into cells. Although viruses offer the greatest efficiency, they also raise major safety concerns including cancer and inflammation. A safer alternative is the use of ultrasound together with small bubbles (micro bubbles) that enhance the uptake of drugs and genes into cells by disrupting the cell membrane in a reversible way. We hypothesized that ultrasound could be used to guide local microbubble-enhanced delivery of DNA. In the study we tested various parameters of ultrasound force, concentration of DNA, number of treatments and more in order to establish the optimal methodology for gene delivery. Our findings defined certain parameters that showed the highest gene delivery efficiency without damaging the tissue.