This study demonstrates how it is possible to correct precisely the mutations that exist in patients with genetic diseases. In the work, we focused on two specific patients who have different forms of limb girdle muscular dystrophy. For each patient, we used the CRISPR/Cas9 genome editing method to change the mutated base in the patient’s DNA to the correct base. CRISPR/Cas9 is a recently-developed genetic engineering method that allows a researcher to make precise changes in the DNA of the chromosomes. For one of the patients, we also applied two other methods, called DICE and THRIP, to add a healthy version of the mutated gene to a safe location in the patient’s chromosomes. These alternative strategies are faster and more efficient than the CRISPR/Cas9 method. In all cases, we showed that the corrected cells were now able to make the proteins that were missing or inactive previously in the patient cells. By changing the DNA sequence in stem cells derived from the patients, the resulting corrected stem cells could potentially be used in cell therapy strategies to improve the clinical condition of these patients.