From MEFs to Matrigel 2: splitting hESCs from MEFs onto Matrigel.

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Publication Year:

2008

Authors:

Ivan Khvorostov, Jin Zhang, Michael Teitell

PubMed ID:

19066543

Funding Grants:

Role of Mitochondria in Self-Renewal Versus Differentiation of Human Embryonic Stem Cells

Public Summary:

This video demonstrates how to grow human embryonic stem cells (hESCs) on mouse embryonic fibroblast (MEF) feeder cells, how to passage hESCs from MEF plates to feeder cell-free Matrigel plates.

Scientific Abstract:

This video demonstrates how to grow human embryonic stem cells (hESCs) on mouse embryonic fibroblast (MEF) feeder cells, how to passage hESCs from MEF plates to feeder cell-free Matrigel plates.